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Research Associate II - CAR T Cell Therapeutics Research

City of Hope

Duarte, CA

Job posting number: #7092246 (Ref:10014790)

Posted: January 14, 2022

Application Deadline: Open Until Filled

Job Description

About City of Hope

City of Hope, an innovative biomedical research, treatment and educational institution with over 6000 employees, is dedicated to the prevention and cure of cancer and other life-threatening diseases and guided by a compassionate, patient-centered philosophy.

Founded in 1913 and headquartered in Duarte, California, City of Hope is a remarkable non-profit institution, where compassion and advanced care go hand-in-hand with excellence in clinical and scientific research.  City of Hope is a National Cancer Institute designated Comprehensive Cancer Center and a founding member of the National Comprehensive Cancer Network, an alliance of the nation’s leading cancer centers that develops and institutes standards of care for cancer treatment.

Position Summary
A Research Associate II position is available in the CAR T Cell Therapeutics Research Laboratory.  This is a fast-paced lab involved in cutting edge technology, research and clinical immunotherapeutic programs.  We will train new personnel in project specific techniques and procedures.  Work hours may vary. Please apply online for consideration.  

Key Responsibilities include:

  • Tissue culture including application of aseptic techniques for media compounding, passaging, maintenance and cryopreservation of cell cultures (e.g., T cell lines)
  • Human iPSC generation, iPSC routine culture, colonization and differentiation culture into T cells
  • CRISPR gene editing on iPSC cells and T cells.  Flow cytometric characterization of T cell, tumor cell lines and iPS cells
  • Performance of T cell functional assays (e.g., cytotoxicity and cytokine production assays) and ELISAs
  • Murine surgical procedures and tissue harvesting/preparation
  • Assisting in revising/maintaining lab protocols
  • Providing routine lab support and maintenance activities in the spirit of GLP.  

Basic education, experience and skills required for consideration:

  • Requires a Bachelor’s degree in Biological Sciences or a related field, plus 2 years of research experience; or 1 year of experience with a Master’s degree.
  • Meticulous attention to detail and accuracy in lab work as well as accurate recordkeeping and lab documentation are a must.

Preferred education experience and skills:

  • Master's degree
  • Hands-on experience with cellular immunology and standard good laboratory practices (GLP) desired
  • Priority will be given to candidates with previous experience in in human iPSC culture, reprogramming and differentiation culture techniques

Additional Information:

  • See the lab web page for an overview of research interests at: https://www.cityofhope.org/people/forman-stephen
  • Please note, there are multiple openings available.
  • To protect the health of patients and staff and to comply with new State of California mandates, City of Hope staff are required to show proof of full vaccination by September 30, 2021.  Compliance is a condition of employment.  

City of Hope is committed to creating a diverse environment and is proud to be an equal opportunity employer.  All qualified applicants will receive consideration for employment without regard to race, religion, color, national origin, sex, sexual orientation, gender identity, age, status as a protected veteran, or status as a qualified individual with disability.



City of Hope is a community of people characterized by our diversity of thought, background and approach, but tied together by our commitment to care for and cure those with cancer and other life-threatening diseases. The innovation that our diversity produces in the areas of research, treatment, philanthropy and education has made us national leaders in this fight. Our unique and diverse workforce provides us the ability to understand our patients' needs, deliver compassionate care and continue the quest for a cure for life-threatening diseases. At City of Hope, diversity and inclusion is a core value at the heart of our mission. We strive to create an inclusive workplace environment that engages all of our employees and provides them with opportunities to develop and grow, both personally and professionally. Each day brings an opportunity to strengthen our work, leverage our different perspectives and improve our patients’ experiences by learning from others. Diversity and inclusion is about much more than policies and campaigns. It is an integral part of who we are as an institution, how we operate and how we see our future.


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More Info

Job posting number:#7092246 (Ref:10014790)
Application Deadline:Open Until Filled
Employer Location:City of Hope
Duarte,California
United States
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