Job DescriptionROLE SUMMARY
The Viral Vaccine group is seeking for a motivated Sr. Associate Scientist with a strong understanding and experience in DNA synthesis and molecular biology contribute to help optimize vaccine design strategies in viral vaccine development. The incumbent is responsible for supporting the preclinical evaluation and early clinical development of our RNA-based vaccine platform. The incumbent will support DNA synthesis, molecular cloning, and construct characterization. In particular, the incumbent will assist in the implementation of novel synthetic technology and molecular biology approaches that can rapidly, accurately, and efficiently produce high-quality DNA constructs to make game-changing RNA vaccines with superior efficacy or for other virology applications. Work and documentation are to be done in a compliant manner according to relevant SOP guidelines and cGLP guidelines where appropriate. The incumbent will be part of a dynamic team and should possess a strong work ethic and collaborative spirit.
Apply gene synthesis technologies and molecular biology to advance our RNA-based vaccine platform technology, include (but are not limited to) molecular biology work for the annual strain change for Influenza virus and new emerging variant of SARS-CoV-2.
Perform molecular biology techniques that include recombinant DNA techniques, sequence analysis, PCR, restriction digestion, ligation/Gibson Assembly/ Golden Gate assembly, miniprep, maxiprep, DNA/RNA concentration determine, agarose gel analysis, RT-qPCR/ddPCR, RNA manipulation and analysis.
Support the development and implementation of molecular tools and assays to characterize DNA/RNA.
In collaboration with manager, interpret and summarize experimental data. Present experimental results at group meetings as needed.
Generate and characterize reagents used to perform necessary assays and support maintenance of laboratory supplies.
Regular review of literature to maintain knowledge of scientific trends and industry processes, advance molecular biology, and RNA-based vaccine.
Maintain laboratory notebooks in compliance with the Pfizer Laboratory Notebook Policy.
Performs other duties as assigned.
Satisfactorily completes all safety and quality training in conformance with Departmental requirements.
Degree in Molecular Biology, Genetics, Virology, or a related scientific field. MS with 0-2 years of research experience, or BS with +3 years of laboratory research experience.
Laboratory hands-on experience with standard molecular biology techniques (PCR/qPCR, cloning, DNA/RNA production and purification, sanger sequencing and analysis, etc.)
Technical expertise of molecular biology and sequence analysis software/webserver.
Must be detail oriented; possess problem solving skills, and the ability to handle multiple tasks. Adaptability to changing priorities and projects is expected.
Must possess good interpersonal and communication skills and be a team player in a cross-functional collaborative environment.
Direct knowledge of influenza virus strain change is highly preferred.
Previous experience handling and manipulating DNA synthesis preferred.
Previous hands-on experience with automation of DNA synthesis or Sanger Sequencing preferred.
Industry experience preferred.
Strong laboratory training in aseptic technique is essential. Ability to work with infectious agents safely. The capacity to work effectively in a highly visible research program with time-constrained milestones.
Pfizer is committed to equal opportunity in the terms and conditions of employment for all employees and job applicants without regard to race, color, religion, sex, sexual orientation, age, gender identity or gender expression, national origin, disability or veteran status. Pfizer also complies with all applicable national, state and local laws governing nondiscrimination in employment as well as work authorization and employment eligibility verification requirements of the Immigration and Nationality Act and IRCA. Pfizer is an E-Verify employer.