Job Description

Job Summary
A research technician position is available in the Maurer Lab at the University of Michigan. The Maurer Lab combines super-resolution microscopy with genomic, biochemical, and proteomic approaches to understand the vector-host interactions driving successful rAAV gene therapies. Read more about our research here: www.maurerlab.org


We are particularly interested in a candidate who has experience with AAV Vectors, mammalian cell culture, and molecular cloning techniques. The Maurer lab is part of the Department of Biophysics, which has a long tradition of education and research in the field. The University of Michigan Department of Biophysics has pushed the boundaries of traditional work in fundamental chemical sciences into the interface with other disciplines.

Responsibilities*
To prepare and apply rAAV vector stocks to cultured cells
Collect data via microscopy, qPCR, and other methods
Clone plasmids according to experimental needs.
Additionally, this person will have the opportunity to gain experience and training in communicating their work outside of the laboratory through peer-reviewed publications and conference presentations
Work independently in tissue culture and on benchwork under general supervision
Optimize or troubleshoot AAV transduction and production protocols
Perform live and fixed cell microscopy
Establish high-content imaging workflows and perform data collection
Analyze and interpret data from above protocols, including high-content imaging pipeline development
Maintain detailed records in LabArchives
Ensure quality control over vector titration reagents and results
Contribute intellectually to experimental planning
Required Qualifications*
Molecular and cellular biology-related BS degree that includes 2+ years of wet lab experience.
Expertise in mammalian cell culture and sterile technique.
Experience with multichannel pipetting and 96-well plate assays, including use of fluorescence and luminescence plate readers.
Ability to read, interpret, and critically evaluate primary scientific literature.
Experience with molecular cloning, including Gibson assembly, PCR, restriction digestions, and ligation
Proficiency with Microsoft Excel, PowerPoint, word, and some experience with scientific writing (through coursework or otherwise).
Desired Qualifications*
Preferred experience with SnapGene, CellProfiler or CellPose, and LabArchives software
Basic coding skills (vibe coding counts)